Functions and regulation of ß-1,3-glucanases during seed germination, dormancy release and after-ripening

Figure 1.   The effect of abscisic acid (ABA) (A), after-ripening (B,C) and class I ß-1,3-glucanase (ßGlu I) sense and antisense transformation on the kinetics of testa and endosperm rupture during tobacco seed germination. The incidence of testa and endosperm rupture is expressed as the percentage of sense-ßGlu I (TKSG7), antisense-ßGlu I (TKAG4) and empty-vector (TCIB1) seeds scored with time after the start of imbibition in continuous light. (A) The effect of ABA on testa and endosperm rupture of after-ripened seeds incubated in medium without (Control) and with 10 µM ABA added. (B,C) The effect of after-ripening on testa rupture (B) and endosperm rupture (C) investigated by comparing fresh and after-ripened TKSG7, TKAG4 and TCIB1 seeds incubated in control medium. Note that ßGlu I is over-expressed in TKSG7 seeds, but that TKAG4 seeds express TCIB1-like levels of ßGlu I during endosperm rupture. The antisense-ßGlu I transformation therefore only affects testa rupture of TKAG4 seeds and the delay in endosperm rupture is attributed to the delay in testa rupture. Means of single line mean values from several independent lines are presented; the single line mean values ± SE are published in Leubner-Metzger and Meins (2000), Leubner-Metzger and Meins (2001) and Leubner-Metzger (2002).

© Gerhard Leubner-Metzger, Seed Science Research 13: 17-34 (2003)

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