Brassinosteroids promote seed germination (2003)
Gerhard Leubner-Metzger
Universität Freiburg, Institut für Biologie II, Botanik, Schänzlestr.
1, D-79104 Freiburg, Germany Book Chapter 5 in: "Brassinosteroids", Hayat S, Ahmad A (Eds.),
Kluwer Academic Publishers, The Netherlands, pp. 119-128 (2003)
Figure 1. The accumulation of XET enzyme activity in germinating seeds of Nicotiana tabacum cv. Havana 245.
Tobacco seeds were imbibed without (Control; C) and with 10 nM brassinolide (BR) in the medium and incubated for the times indicated (hours) in continuous light; in addition 10 μM ABA was added to the medium of one series (ABA) (Leubner- Metzger, 2001). Protein extracts from entire seeds, seed tissues (Endosperm, Embryo), or seedlings were used. Testa rupture in the populations of ca. 150 seeds was 0 % at 30 h and 100 % at 45 h. Endosperm rupture was 0 % (45 h), ca. 0 % (Control, 55 h) and ca. 30 % (BR, 55 h); only seeds without endosperm rupture were used for the extracts. Tissues were homogenized and XET enzyme activities were determined by the XET 'dot blot' assay as described by Fry (1997). For the semiquantitative XET assay ca. 3 μl (60 μg protein) were applied onto the XET 'dot blot' test paper and incubated for 7 h at 25 °C. Elevated fluorescence under the UV lamp is indicative for accumulating XET enzyme activity.
Key words: abscisic acid - Arabidopsis - brassinosteroids - gibberellins - ß-1,3-glucanase (ßGlu I) - Nicotiana - Orobranche - parasitic angiosperms - seed germination - Striga - xyloglucan endo-transglycosylase (XET)
| Article in PDF format (54 KB) | Abstract Fig. 1 Hyperlink to Planta (2001) |
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