Planta 199: 282-288 (1999)

Effects of gibberellins, darkness and osmotica on endosperm rupture and class I ß-1,3-glucanase induction in tobacco seed germination

Gerhard Leubner-Metzger, Corinne Fründt, Frederick Meins, Jr.

osmotica and tobacco seed germination Fig. 3A-C. The effect the osmoticum and GA4 treatment on the time course of tobacco seed germination and ß-1,3-glucanase activity in seeds incubated in continuous light.

The incidence of seed coat rupture (A) and endosperm rupture (B) expressed in per cent of 100-200 seeds scored.

The activity of ß-1,3-glucanase (C) expressed in pkat/µg protein.

Seeds were incubated without PEG 6000 or GA4, with 0.04 M PEG 6000, and with 0.04 M PEG 6000 + 10-5 M GA4 added at to the medium at the start of the experiment. The data are presented as mean values, usually for at least two samples in two independent experiments. Error bars= maximum ±SE

Article in PDF format (2.5 MB) Abstract          Fig. 1          Fig. 2          Fig. 3          Tab. 1  

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