Plant Molecular Biology 38: 785-795 (1998)

Ethylene responsive element binding protein (EREBP) expression and the transcriptional regulation of class I ß-1,3-glucanase during tobacco seed germination

Gerhard Leubner-Metzger, Luciana Petruzzelli (1), Rosa Waldvogel, Regina Vögeli-Lange (2), Frederick Meins, Jr.

Table 1.  Effect of ethylene (100 ppm) on ß-1,3-glucanase and Glb promoter activity in Glb-GUS tobacco seed

Incubation
conditions
Seed
component 1
ß-1,3-Glucanase
(pkat / seed)
GUS
(fkat / seed)
Endosperm rupture
(%)
    Control Ethylene Control Ethylene Control Ethylene
               
54 h light           0.2 ± 0.2 8.2 ± 0.8
  Embryo 0.12 ±0.002 0.16 ± 0.00 0.02 ± 0.01 0.02 ± 0.00    
  Endosperm 1.22 ± 0.02 2.07 ± 0.11 0.61 ± 0.03 1.59 ± 0.16    
               
65 h light 3              
No addition Entire seed 1.65 ± 0.09 3.00 ± 0.31 1.00 ± 0.14 3.27 ± 0.36 79.3 ± 3.5 87.5 ± 1.6
1 mM AVG Entire seed 0.62 ± 0.07 1.32 ± 0.02 0.25 ± 0.09 0.67 ± 0.00 12.6 ± 4.0 59.1 ± 0.6
50 ppm NBD Entire seed 1.04 ± 0.10 2.43 ± 0.00 0.59 ± 0.05 1.91 ± 0.06 17.2 ± 2.6 62.8 ± 0.4
               
80h darkness           3.5 ± 0.9 4.7 ± 0.7
  Entire dormant seed 0.10 ± 0.01 0.17 ± 0.05 0.02 ± 0.01 0.03 ± 0.00    
  Entire non-dormant seed 1.45 ± 0.31 3.40 ± 0.92 0.30 ± 0.02 0.93 ± 0.19    

1   Batches of 100 dissected or entire seeds were assayed. Samples of embryos and endosperms were obtained from seeds with intact endosperms. Note that dormant seeds have intact testas and intact endosperms.
2   Mean values ± SE for at least two independent experiments.
3   Testa rupture ≥ 96 % in all 65 h light samples.


Hyperlink to drawing of the tobacco class I ß-1,3-glucanase B (GLB) gene promoter


Article in PDF format (2.1 MB) Abstract          Fig. 1          Fig. 2          Fig. 3          Table 1          GLB-promoter  

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