Proteomics 10: 406-416 (2010)

Proteomics reveal tissue-specific features of the cress (Lepidium sativum L.) endosperm cap proteome and its hormone-induced changes during seed germination [W]

Kerstin Müller, Claudette Job, Maya Belghazi, Dominique Job, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de (K.M., G.L.-M.)
Centre National de la Recherche Scientifique-Université Claude Bernard Lyon-Institut National des Sciences Appliquées-Bayer CropScience Joint Laboratory (UMR 5240), Bayer CropScience, Lyon, France (C.J., D.J.)
Centre d'Analyse Protéomique de Marseille, Institut Fédératif de Recherche Jean Roche, Marseille, France (M.B.)

Received July 29, 2009; revised November 3, 2009; accepted November 5, 2009
DOI 10.1002/pmic.200900548

Supplemental Table S3. Identified proteins from the cap of germinating cress seeds belonging to functional category 06–protein folding and stability.

 

fold change

 

 

spot #

8 to
18

18 to
18 ruptured

8 to
18 ABA

18 ABA to
96 ABA

protein name

functional category

17

2.01

0.13

1.51

0.73

SHD (SHEPHERD); HSP 90-like

06.01 folding and stability

23

2.44

1.05

1.88

1.29

protein disulfide isomerase precursor-like

06.01 folding and stability

24

0.89

0.37

1.30

0.57

heat shock cognate protein 80

06.01 folding and stability

276

1.71

1.75

1.07

0.94

chaperonin 10

06. folding and stability

290

0.47

2.53

0.92

1.05

chaperonin 10

06.01 folding and stability

236

2.82

0.77

1.31

1.52

aspartyl protease family protein

06.13 proteolysis

266

0.99

0.76

1.46

3.63

aspartyl protease family protein

06.13 proteolysis

223

1.88

1.30

1.13

1.10

26S proteasome regulatory subunit, putative

06.13 proteolysis

278

3.06

4.60

1.22

1.50

Cruciferin CRU1 precursor (11S globulin)

06. storage protein

301

0.85

2.16

0.67

0.22

CRU3 (CRUCIFERIN 3)

06.20 storage protein

302

0.82

2.32

0.23

2.44

CRU3 (CRUCIFERIN 3)

06.20 storage protein

317

1.37

0.49

1.07

0.62

CRA1 (CRUCIFERIN A)

06.20 storage protein

297

0.33

0.57

0.12

0.91

seed storage protein beta-chain 6 (fragment)

06.20 storage protein


Supplemental Table S3. Identified proteins from the cap of germinating cress seeds belonging to functional category 06–protein folding and stability.
Only spots in which just one protein was identified are listed.
“Fold change” is calculated from the averaged means of normalized spot volumes of three 2D-gels loaded with biological replicates.
Bold numbers indicate statistically significant differences at p<0.05 in a t-test.
For detailed information on spot characteristics, refer to Supplemental Table S1 and Supplemental Fig. S2.

Article in PDF format (365 KB)
Supplemental data file (5 MB)
Abstract

Figure 1         Figure 2         Figure 3         Figure 4
Suppl. Figures:     S1     S2     S3     S4
Suppl. Tables:      S1     S2     S3     S4
 
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http://www.proteomics-journal.com/
 

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