Journal of Experimental Botany 63: 6325-6334 (2012)

Role of a respiratory burst oxidase of Lepidium sativum (cress) seedlings in root development and auxin signalling [W][OA]

Kerstin Müller*, Ada Linkies*, Gerhard Leubner-Metzger, Allison R. Kermode

Simon Fraser University, Department of Biological Sciences, 8888 University Drive, Burnaby BC V5A 1S6, Canada (KM, ARK)
University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, German (AL, GLM)
School of Biological Sciences, Plant Molecular Science, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK (GLM); Web: 'The Seed Biology Place' -
* equal contributions

Received 20 July 2012; Revised 11 September 2012; Accepted 21 September 2012
Advance Acess publication 23 October 2012
DOI 10.1093/jxb/ers284

Fig. 5 rboh

Fig. 5. Expression of auxin-related genes in roots and shoots of L. sativum seedlings of wild-type and Lesarboh-RNAi knockdown lines.
Transcript abundance for the genes encoding the LOBdomain containing protein (LBD) 16 (A), the auxin-responsiveprotein, IAA14 (B) and the auxin-responsive protein IAA5 (C) as determined by qRT-PCR. Stage 1 corresponds to the phenotype shown in Fig. 4B, while stage 2 corresponds to the phenotype shown in Fig. 4C–E. Transcripts in wild-type seedlings were examined under 2,4-D treatment (24 h on 1 μM 2,4-D) and control conditions. Transcript abundance was corrected to the abundance of transcripts associated with the standard genes – the constitutive genes Actin 7 (ACT7; At5g09810) and Elongation Factor 1α (EF1α; At5g60390). Averages of three biological replicates ± SE are presented.

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Fig. S1       Fig. S2
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