Plant, Cell and Environment 26: 661-671 (2003)

Calcium requirement for ethylene-dependent responses involving 1-aminocyclopropane-1-carboxylic acid oxidase in radicle tissues of germinated pea seeds

Luciana Petruzzelli, Monica Sturaro, Davide Mainieri, Gerhard Leubner-Metzger

© Petruzzelli et al. Plant, Cell and Environment 26: 661-671(2003)

Figure 5.  Effects of calcium antagonists on root hair formation and elongation growth of ethylene-treated germinated pea seeds. 48-h imbibed pea seeds were preincubated for 2 h in presence or absence of the calcium antagonists followed by a further incubation for 8 h with exposure to 30 µl/l ethylene in presence or absence of the calcium antagonists. The cell differentiation zone (dz) where the root hairs normally form, the cell elongation zone (ez) where ethylene-induced ectopic root hairs form and the root tip (rt) are indicated. Estimations for the relative root hair amount ('+' = control radicle dz amount, '++' and '+++' = increased amount, '(+)' = slightly decreased amount, '-' = absence of root hairs) and length are presented in the right part of the figure.

(A) Control radicle without ethylene treatment; Note that root hairs are present in the dz, but not in ez, and are only visible in the 8 h-incubated radicles at higher magnification.
(B) Ethylene-treated radicle with ectopic root hair formation.
(C) Ethylene plus 300 µl/l NBD (2,5-norbornadiene).
(D) 10 mM CaCl2 without ethylene treatment.
(E) Ethylene plus 10 mM CaCl2.
(F) Ethylene plus 1 mM LaCl3.
(G) Ethylene plus 50 µM RR.
(H) Ethylene plus 50 mM LiCl.
(I) Ethylene plus 50 mM LiCl plus 10 mM CaCl2.

cover pea Cover photograph of the May 2003 issue of Plant, Cell & Environment related to
this publication showing the effect of ethylene treatment on pea radicle growth

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Article in PDF format (332 KB)
Abstract          Fig. 1          Fig. 2          Fig. 3          Fig. 4          Fig. 5          ßGlu expression
© Blackwell Science

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