Plant, Cell and Environment 26: 661-671 (2003)

Calcium requirement for ethylene-dependent responses involving 1-aminocyclopropane-1-carboxylic acid oxidase in radicle tissues of germinated pea seeds

Luciana Petruzzelli, Monica Sturaro, Davide Mainieri, Gerhard Leubner-Metzger

pea ACO seed geermination

© Petruzzelli et al. Plant, Cell and Environment 26: 661-671(2003)

Figure 3. Effects of calcium antagonists on the regulation by ethylene of Ps-ACO1 and Ps-ACS2 transcript levels in the embryonic axes of germinated pea seeds.

The 48 h-imbibed pea seeds were pre-incubated for 2 h in the presence or absence of the calcium antagonists followed by a further incubation for 8 h with 30 mL/L ethylene in presence or absence of the calcium antagonists. Subsequent RNA blot analyses was as described in Fig. 1.

The effects of the following substances were investigated, as indicated:
(a) different CaCl2 concentrations without ethylene treatment.
(b) Ethylene plus the membrane-impermeable Ca2+ chelator EGTA (5 mM) or the Ca2+ channel inhibitors LaCl3 (1 mM) and verapamil (Ver, 100 mM).
(c) Ethylene plus the Ca2+ channel inhibitor ruthenium red (RR).
(d) Ethylene plus the CaM-binding inhibitor W-7.
(e, f) Ethylene plus the inhibitors of the PI cycle neomycin and LiCl.

A representative experiment from three independent experiments with the same result is presented

cover pea Cover photograph of the May 2003 issue of Plant, Cell & Environment related to
this publication showing the effect of ethylene treatment on pea radicle growth

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Abstract          Fig. 1          Fig. 2          Fig. 3          Fig. 4          Fig. 5          ßGlu expression
© Blackwell Science

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