Seed Science Research 13: 139-153 (2003)

Distinct expression patterns of ß-1,3-glucanases and chitinases during the germination of Solanaceous seeds

Luciana Petruzzelli, Kerstin Müller, Katrin Hermann, Gerhard Leubner-Metzger

gklucanase Nicotians plumbaginifolia

Figure 2. Tissue specificity and regulation by abscisic acid (ABA) of ßGlu I and Chn I in imbibed seeds and in seedlings of Nicotiana tabacum.

(A) RNA-blot hybridization of total RNA (25 μg/lane) prepared from seeds prior to endosperm rupture (55 h) during imbibition in continuous light and from 90 h seedlings. The RNA-blot was hybridized with a cDNA probe for tobacco ßGlu I, and a probe for 18S rRNA was used as a loading standard. Em = embryo; ME = micropylar endosperm; LE = lateral endosperm; S = shoot; R = root; E = 90 h endosperm remnant.

(B) Immunoblot analysis of seed and seedling extracts (80 μg protein/lane) probed with the rabbit anti-tobacco Chn I antibody. Seeds and seedlings were imbibed in continuous light either in the absence (Control) or presence of 10 μM ABA. CHN I = 10 ng of the authentic 32 kDa and 34 kDa tobacco enzymes.

(C) RNA-blot hybridization of total RNA prepared from seeds and seedlings incubated in medium without (Con) or with 10 μM ABA added (ABA). The RNA-blot was hybridized with probes for tobacco Chn I and 18S rRNA as described in (A).

Article in PDF format (780 KB)
Abstract               Fig. 1          Fig. 2          Fig. 3          Fig. 4     
                            Table 1          Table 2          Table 3
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