Seed Science Research 13: 139-153 (2003)

Distinct expression patterns of ß-1,3-glucanases and chitinases during the germination of Solanaceous seeds

Luciana Petruzzelli, Kerstin Müller, Katrin Hermann, Gerhard Leubner-Metzger

Capsicum annuum

Figure 4. Tissue specificity and regulation by abscisic acid (ABA) of ßGlu antigens during germination of Capsicum annuum seeds, in

(A) embryo and 160 h-seedling,

(B) micropylar cap and

(C) the rest of the seed.

Immunoblot analysis of seed and seedling extracts (40 μg protein/lane) probed with the rabbit anti-tobacco ßGlu I antibody, which recognizes all known tobacco ß-1,3-glucanases and is also known to cross-react with tomato ßGlu I (Neuhaus et al., 1992; Beffa et al., 1993; Petruzzelli et al., 1999; Wu et al., 2000; Kunz et al., 2001). No signals were obtained in control blots with rabbit anti-tobacco Chn I antibody or with rabbit pre-immune serum. Extracts and germination are as described in Fig. 3. R = root, S = shoot.

Article in PDF format (780 KB)
Abstract               Fig. 1          Fig. 2          Fig. 3          Fig. 4     
                            Table 1          Table 2          Table 3
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