Journal of Experimental Botany 61: 491-502 (2010)

Peroxidases identified in a subtractive cDNA library approach show tissue-specific transcript abundance and enzyme activity during seed germination of Lepidium sativum [W][OA]

Ada Linkies, Uta Schuster-Sherpa, Stefanie Tintelnot, Gerhard Leubner-Metzger, Kerstin Müller

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place'

Received August 27, 2009; revised October 6, 2009; accepted October 12, 2009
Advance Acess publication 30 October 2009
DOI 10.1093/jxb/erp318

Linkies JEB Fig. 1
Figure 1. Peroxidase activity histostain of longitudinal sections of Lepidium sativum (cress) seeds after 16 h of imbibition.

(A) longitudinal section of cress embryo and seed covering layers (endosperm, testa). After 2 min in the staining solution, the micropylar endosperm and the radicle display staining on the cut surface.

(B) Longitudinal section of a cress endosperm separated from the testa after 15 min of staining. Cell outlines can be observed over the whole endosperm surface, and the cut surface is stained completely.

(C) Longitudinal section of a cress embryo. After 8 min staining, the colour spread over the whole radicle, but not the cotyledons. ‘cap’ = micropylar endosperm cap.

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Figure 1         Figure 2         Figure 3
Table 1          Table 2          Suppl. data file
Suppl. Figure S1
Suppl. Table S1
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