Plant Physiology 150: 1855-1865 (2009)

In vivo cell wall loosening by hydroxyl radicals during cress (Lepidium sativum L.) seed germination and elongation growth  [W][OA]

Kerstin Müller, Ada Linkies, Robert A.M. Vreeburg, Stephen C. Fry, Anja Krieger-Liszkay, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' (K.M., A.L., G.L.-M.)
The Edingburgh Cell Wall Group, Institute of Molecular Plant Sciences, The University of Edinburgh, The King's Buildings, Mayfield Road, Edinburgh EH9 3JH, United Kingdom (R.A.M.V., S.C.F.)
Commissariat à l'Energie Atomique (CEA), iBiTecS, CNRS URA 2096, Service de Bioénergétique, Biologie Structurale et Mécanisme, 91191 Gif-sur-Yvette Cedex, France (A.K.-L.)

Received April 9, 2009; accepted May 29, 2009; published June 3, 2009

Fig. 3

Figure 3.   Detection of in vivo •OH attack on cress seed radicle cell walls by 3H-fingerprinting (Fry et al., 2001). (A) Representative 3H-fingerprints of acidic products from radicle samples. Signal intensity in the scintillation count is plotted against distance from the origin after high voltage paper electrophoresis (PE) at pH 3.5. Monosaccharide markers were run with the samples. (B) Representative 3H-fingerprints of neutral products from radicle samples. Neutral material (that which co-migrated with glucose during PE) was eluted and re-run by paper chromatography (PC) in an acidic solvent. Peaks N1 and N2 remain unidentified: they did not co-migrate exactly with any monosaccharide tested, and when eluted and re-run in a basic solvent, they migrated within the disaccharide zone (more slowly than the slowest monosaccharide, data not shown). They may therefore have been disaccharides containing unusual sugar residues not susceptible to Driselase digestion. Acidic markers: GalO = galactonic acid, GalA = galacturonic acid, GlcA = glucuronic acid. Neutral markers: Gal = galactose, Glc = glucose, Gal-ol = galactitol, Ara = arabinose, Xyl-ol = xylitol, Xyl = xylose, Fuc = fucose, Rib = ribose.
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