Plant & Cell Physiology 53: 81-95 (2012)

Myrigalone A inhibits Lepidium sativum seed germination by interference with gibberellin metabolism and apoplastic superoxide production required for embryo extension growth and endosperm rupture[W]

Krystyna Oracz*, Antje Voegele, Danuše Tarkowská, Dominique Jacquemoud, Veronika Turecková, Terezie Urbanová, Miroslav Strnad, Elwira Sliwinska, Gerhard Leubner-Metzger*
*shared corresponding authors
University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' - www.seedbiology.de (K.O., A.V., D.J., G.L.-M.)
Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, v.v.i., Šlechtitel? 11, CZ-783 71, Olomouc, Czech Republic (D.T., V.T., T.U., M.S.)
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Šlechtitel? 11, CZ-783 71, Olomouc, Czech Republic (M.S.)
Laboratory of Molecular Biology and Cytometry, Department of Plant Genetics and Biotechnology, University of Technology and Life Sciences, Kaliskiego Ave. 7, PL-85-789 Bydgoszcz, Poland (E.S.)

Received 11 July 2011; Accepted 3 September 2011; First published online 8 September 2011
DOI 10.1093/pcp/pcr124

Supplementary Table S4    Main characteristics of genes and primer sequences for qRT-PCR used in the present work.

Abbreviation of gene

Description of gene

Specificity of primer

Accession number

Forward Primer: sequence 5' --> 3'

Reverse Primer: sequence 5' --> 3'

SDR1/ABA2

cytosolic short-chain dehydrogenase/reductase

A. thaliana

At1g52340

F : ATGTGGAGCACCGTGCCCTG

R : AACGCCTCCCACAACACCTCC

LesaCYP707A2

ABA 8'-hydroxylase CYP707A2

L. sativum

GQ221028 (At2g29090)

F : AAGAGCTTTCATGCCGGATTC

R : AAGAGCTTTCATGCCGGATTC

LesaCYP707A3

ABA 8'-hydroxylase CYP707A3

L. sativum

GQ221029 (At5g45340)

F : ATCAACACCCTCGAACACATG

R : TCAATTTCAGTGGCCTCCTCTT

LesaGID1a

GA receptor GIBBERLLIN INSENSITIVE DWARF 1a

L. sativum

HQ003455 (At3g05120)

F: AGCGGGTGAGTCTGGAATCAAT

R: AAGTTTCTCAGACTCTGTTCTC

LesaGID1b

GA receptor GIBBERLLIN INSENSITIVE DWARF 1b

L. sativum

HQ003456 (At3g63010)

F: AGCTCACAATGTTGCTGTGAG

R:  TCAGTCCTCTCTTGTCCAC

LesaGID1c

GA receptor GIBBERLLIN INSENSITIVE DWARF 1c

L. sativum

HQ003457 (At5g27320)

F: GCATAATGTCGCGGTTAGAG

R: GTCCCTCCAAACATAGGGTT

GA3ox1

GA3 oxidase 1

A. thaliana

At1g15550

F: TGGGCCCAAGCTGCTCTCCA

R: AGCCAGGAAACGGTGGCACG

GA3ox2

GA3 oxidase 2

A. thaliana

At1g80340

F: GATGGACGTGGGCTGGGTTA

R: CCCTGGCGGTGAAGCACG

GA3ox3

GA3 oxidase 3

A. thaliana

At4g21690

F: AACCCCGTGAACCGTGACCG

R: ACGGGAATGGGTCCGCTCGT

GA3ox4

GA3 oxidase 4

A. thaliana

At1g80330

F: GTGGGCAGAGGCGCCATACG

R: CCGGCTCAACCGTGACCCAA

GA2ox7

GA2 oxidase7

A. thaliana

At1g50960

F: GCAGCTGCGAAAGAGTGGGGA

R: TGAGCGGGAGAAGTGGCGCT

Lesa17210

IPL1; IAP-like protein 1, zinc ion binding

L. sativum

HQ912755 (At1g17210)

F: TCCGCCCTTGTATGGACGAGA

R: CTCTGGCACCTGGGAAAGCCA

Lesa20000

HBT; anaphase-promoting complex subunit

L. sativum

HQ912757 (At2g20000)

F: TCTGGTCCACGACGGAGCTTG

R: TCTGGTCCACGACGGAGCTTG

Lesa04660

APC2; ubiquitin-protein ligase cell cycle regulation

L. sativum

HQ912754 (At2g04660)

F: AGCTGGGTCTATTGCACGAAG

R: AGCTGGGTCTATTGCACGAAG


Article in PDF format (1.5 MB)
Supplementary data file (3.4 MB)
Abstract
Supplementary:
Fig. 1         Fig. 2         Fig. 3         Fig. 4         Fig. 5         Fig. 6       Table 1
Fig. S1       Fig. S2       Fig. S3       Fig. S4       Tab.S1     Tab.S2     Tab.S3     Tab.S4
 
© Oxford University Press, Oxford Journals
Japanese Society of Plant Physioloists
http://pcp.oxfordjournals.org/
 

The Seed Biology Place
Copyright © Gerhard Leubner 2000
Contact: gerhard.leubner@biologie.uni-freiburg.de

Webdesign Gerhard Leubner 2000
Best viewed with browser version 4 and 800x600 pixel
This page was last updated on 18 January, 2012