Journal of Experimental Botany 62: 5131–5147 (2011)

Members of the gibberellin receptor gene family GID1 (GIBBERELLIN INSENSITIVE DWARF1) play distinct roles during Lepidium sativum and Arabidopsis thaliana seed germination [W][OA]

Antje Voegele, Ada Linkies, Kerstin Müller*, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place'
*Department of Biological Sciences, Simon Fraser University, 9 8888, University Drive, Burnaby BC, V5A 1S6, Canada

Received 14 March 2011; Revised 22 May 2011; Accepted 13 June 2011
Advance Acess publication 21 June 2011
DOI 10.1093/jxb/err214

GID1 transcript abundance Arabidopsis seeds

Fig. 2. Transcript abundances in whole seeds of the three Arabidopsis thaliana GID1 genes during germination.

A, The time course of endosperm rupture of A. thaliana (Col) seeds on medium without (CON) or with 10µM GA4+7 (GA) or 1µM ABA added. After-ripened seeds were incubated at 24 ºC in continuous light without preceding stratification. Mean values ± SE of 3x 50 seeds.

B, In silico analysis on GID1 transcript abundance during early germination of unstratified Arabidopsis (Col) seeds based on eFP-Browser microarray data; Relative expression values ± SD for AtGID1a (At3g05120), AtGID1b (At3g63010) and AtGID1c (At5g27320).

C, GID1 transcript abundance pattern determined by qRT-PCR in whole seeds of Arabidopsis (Col) at 30h, i.e. at 0% endosperm rupture for CON (see panel A). ddCt expression values relative to validated constitutive transcripts are presented. Medium additions as in A; Mean values ± SE of 4x >1000 seeds.

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