Journal of Experimental Botany 62: 5131–5147 (2011)

Members of the gibberellin receptor gene family GID1 (GIBBERELLIN INSENSITIVE DWARF1) play distinct roles during Lepidium sativum and Arabidopsis thaliana seed germination [W][OA]

Antje Voegele, Ada Linkies, Kerstin Müller*, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place'
*Department of Biological Sciences, Simon Fraser University, 9 8888, University Drive, Burnaby BC, V5A 1S6, Canada

Received 14 March 2011; Revised 22 May 2011; Accepted 13 June 2011
Advance Acess publication 21 June 2011
DOI 10.1093/jxb/err214

Arabidopsis gid1 mutant seed germination

Fig.4. The effect of the three GID1 receptors on the seed germination of Arabidopsis thaliana studied with mutants for AtGID1a, AtGID1b and AtGID1c.

Time-course analysis of endosperm rupture of after-ripened seeds of Arabidopsis single (gid1a, gid1b, gid1c) and double (gid1agid1b, gid1bgid1c, gid1agid1c) mutants imbibed at 24ºC in continuous light without preceding stratification. The results presented were obtained with the mutant set by Iuchi et al. (2007), the same results were obtained with an independent mutant set by Willige et al. (2007).

A, Medium without hormones added (control, CON).
B, 10µM GA4+7 (GA)
C, 1µM ABA.
D, Tetrazolium-assay to confirm viability of gid1agid1c; (-) and (+): negative (seed killed by preceding heat treatment, 1 h at 100 ºC) and positive wild-type seed controls. Mean values ± SE of 3x 50 seeds are presented.

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